Conjugation

Part:BBa_K1053124

Designed by: Yasuhito Ito   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)

Split EYFP C-terminal domain fused with λN peptide

λN peptide was fused to the split EYFP C-terminal domain via a flexible 10 residues linker (Gly-Ser-Ser-Gly-Ser-Ser-Gly-Ser-Gly-Ser)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None